cytotoxicity evaluation of proroot mta, root mta and portland cement on human gingival fibroblasts

introduction: the aim of this study was to compare the cytotoxicity of proroot mta (pmta), root mta (rmta) and portland cement (pc) on human gingival fibroblasts (hgfs). materials and methods: the extracts of the test materials were placed close to human gingival fibroblasts (hgfs) within 96-well plates. cellular viability was assessed using mtt assay in different intervals (freshly mixed, 4, 24, and 168 hours after mixing). the data were analyzed using the one-way anova and tukey’s test at 95% significance level. results: it was indicated that there was not a significant difference in cytotoxicity of test materials (p>0.05). in addition, there was not a statistically significant difference between different time intervals within each group (p>0.05). conclusion: pmta, rmta and pc showed comparative biocompatibility while evaluated in-vitro .